A method for manufacturing enzyme granules, and enzyme granules (2024) Palmunen K, Lehtikari L, Perkkalainen M. WO2024134012.
Phytase variants with improved stability and IP4 activity (2023). Juntunen K, Ahola P, Jokinen L, Metzger T, Kühn I, Puranen T, Paloheimo M. WO2023285737.
Phytase variants with improved stability and IP4 activity (2023). Juntunen K, Ahola P, Benson S, Lorenz L, Metzger T, Kühn I, Puranen T, Paloheimo M. WO2023285736.
Phytase variants with improved stability and IP4 activity (2023). Juntunen K, Ahola P, Jokinen L, Metzger T, Kühn I, Puranen T, Paloheimo M. WO2023285735.
Xylanase variants (2022) Paloheimo M, Juntunen K, Turunen O, Ahola P, Taipalus P, Müller E, Savolainen J, Langfelder K, Puranen T. WO2022144500.
Improved mannanase variants (2021). Juntunen K, Valtakari L, Mäkinen S, Oja M, Hellmuth H, Ojapalo P, Langfelder K, Puranen T. WO2021001595.
Solution stable enzyme composition(2020) Palmunen K, Besenmatter W, Perkkalainen M, Lehtikari L. WO2020208296.
Polypeptides having phytase activity (2020). Paloheimo M, Oja M, Scholten S, Löbel D, Juntunen K, Meyerjurgens S, Meriläinen HM, Haarman T, Kühn I, Puranen T. WO2020128152.
Fusion protein (2020). Juntunen K, Valtakari L, Mäkinen S, Puranen T. WO2020099720.
Fungal cellulase variants with improved stability (2020). Juntunen K, Valtakari L, Mäkinen S, Alapuranen M, Hellmuth H, Maike A, Mennicken M, Schwaneberg U, Schönauer D, Ojapalo P, Puranen T. WO2020099719.
Method of precipitating phytase (2020). Palmunen K, Perkkalainen M, Lehtikari L, Kühn I. WO2020094922.
Variants of fungal cellulase (2019). Juntunen K, Valtakari L, Mäkinen S, Alapuranen M, Hellmuth H, Ojapalo P, Mennicken M, Schwaneberg U, Schönauer D, Puranen T. WO2019122520.
Mannanase variants (2018). Leinonen T, Valtakari L, Seefried M, Juntunen K, Puranen T. WO2018220274.
Detergent compositions comprising fungal mannanases (2018). Herbst D, Wieland S, Mussmann N, Leinonen T, Valtakari L, Rachinger M, Juntunen K, Vehmaanperä J, Ojapalo P, Puranen T, Järvinen K, Mäkinen S, Paloheimo M, Kallio J. EP3385362.
Bacterial mannanases (2018). Leinonen T, Valtakari L, Rachinger M, Juntunen K, Järvinen K, Dollak D, Lorenz P, Vehmaanperä J, Ojapalo P, Puranen T, Herbst D, Wieland S, Mussmann N. WO2018185367.
Fungal mannanases (2018). Leinonen T, Valtakari L, Rachinger M, Juntunen K, Järvinen K, Mäkinen S, Paloheimo M, Kallio J, Vehmaanperä J, Ojapalo P, Puranen T, Dollak D, Lorenz P, Herbst D, Wieland S, Mussmann N. WO2018185366.
Detergent compositions comprising bacterial mannanases (2018). Herbst D, Wieland S, Mussmann N, Leinonen T, Valtakari L, Rachinger M, Juntunen K, Dollak D, Lorenz P, Vehmaanperä J, Ojapalo P, Puranen T, Järvinen K. WO2018184767.
Methods for controlling protease production (2016). Paloheimo M, Mäkinen S, Punt P, Juntunen K, Puranen T, Vehmaanperä J. WO2016132021.
Method for concentrating proteins (2016). Palmunen K, Perkkalainen M. WO2016097484.
Fungal endoglucanase variants, their production and use (2016). Juntunen K, Alapuranen M, Valtakari L, Meriläinen H-M, Puranen T. WO2016066896.
Novel esterases in the treatment of cellulosic and lignocellulosic material (2014). Järvinen K, Juntunen K, Leinonen T, Komander A, Langfelder K, Vehmaanperä J, Puranen T. WO2014064331.
Endoglucanases for Treatment of Cellulosic Material (2014). Leinonen T, Komander A, Langfelder K, Vehmaanperä J, Puranen T. US2014051128.
Novel Proteins for the Treatment of Cellulosic Material (2013). Mäkinen S, Juntunen K, Komander A, Langfelder K, Vehmaanperä J, Puranen T. WO2013182740.
Process of lysing yeast cell walls (2013). Marschner V, Alapuranen M, Vehmaaperä J, Titze K, and Langfelder K. WO2013010937.
Methods for treating cellulosic material and CBHII/Cel6A enzymes useful therein (2011).Puranen T, Kyttänen S, Langfelder K, Vehmaanperä J. WO2011080317.
Immunoglobuliinien puhdistus- ja erotusmenetelmä (2010). Palmunen K, Uotila S, Visuri K. FI2008064.
Fungal endoglucanases, their production and use (2010). Valtakari L, Alapuranen M, Szakacs G, Kallio J, Ojapalo P, Vehmaanperä J, Puranen T. WO2010076388.
Fungal endoglucanases, their production and use (2010).Puranen T, Valtakari L, Alapuranen M, Szakacs G, Ojapalo P, Vehmaanperä J. WO2010076387.
Use of pectinolytic enzymes for the treatment of fruit and vegetable mash and enzyme sequences thereof (2009). Puranen T, Seiboth B, Milos K, Theiss W, Kallio J, Kubicek C, Vehmaanperä J. WO2009141156.
Enzyme fusion proteins and their use (2007).Alapuranen M, Valtakari L, Kallio J, Ojapalo P, Vehmaanperä J. WO 2007118935.
Novel enzymes (2007). Valtakari L, Alapuranen M, Hooma S, Siika-aho M, Kallio J, Viikari L, Ojapalo P, Vehmaanperä J. WO2007071820.
Treatment of cellulosic material and enzymes useful therein (2007). Vehmaanperä J, Alapuranen M, Puranen T, Siika-aho M, Kallio J, Hooman S, Voutilainen S, Halonen T, Viikari L. WO2007071818.
Novel laccase enzymes and their uses (2006). Paloheimo M, Puranen T, Valtakari L, Kruus K, Kallio J, Mäntylä A, Fagerström R, Ojapalo P, Vehmaanperä J. WO2006032724.
Novel laccase enzyme and use thereof (2006). Paloheimo M, Valtakari L, Puranen T, Kruus K, Kallio J, Mäntylä A, Fagerström R, Ojapalo P, Vehmaanperä J. WO2006032723.
Improved cellulases (2006). Vehmaanperä J, Puranen T, Valtakari L, Kallio J, Alapuranen M, Paloheimo M, Ojapalo P. WO2006117432.
Method and DNA constructs for increasing the production level of carbohydrate degrading enzymes in filamentous fungi (2005). Paloheimo M, Mäntylä A, Leskinen S, Fagerström R, Kallio J, Puranen T, Lantto R, Suominen P. WO2005100557.
Method for crystallization of proteins using polysaccharides (2005). Visuri K, Uotila S, Palmunen K. WO2005073245.
SCIENTIFIC ARTICLES
Puranen T, Alapuranen M, Vehmaanperä J. (2014) Trichoderma enzymes for textile industries. In Biotechnology and Biology of Trichoderma. Eds. Gupta V J, Schmoll M, Herrera-Estrella A, Upadhyay R S, Druzhinina I, Tuohy M G. Elsevier, B V, Amsterdam, NL, pp. 351-362.
Ries L, Belshaw NJ, Ilmén M, Penttilä ME, Alapuranen M, Archer DB (2014) The role of CRE1 in nucleosome positioning within the cbh1 promoter and coding regions of Trichoderma reesei. Appl Microbiol Biotechnol. 2014 Jan;98(2):749-62. doi: 10.1007/s00253-013-5354-3. Epub 2013 Nov 17.
Kemppainen K, Ranta L, Sipilä E, Östman A, Vehmaanperä J, Puranen T, Langfelder K, Hannula J, Kallioinen A, Siika-aho M, Sipilä K, von Weymarn N. (2012) Ethanol and biogas production from waste fibre and fibre sludge – The FibreEtOH concept. Biomass and Bioenergy 46: 60-69.
Zhang J, Siika-aho M, Puranen T, Tang M, Tenkanen M, Viikari L. (2011) Thermostable recombinant xylanases from Nonomuraea flexuosa and Thermoascus aurantiacus show distinct properties in the hydrolysis of xylans and pretreated wheat straw. Biotechnology for Biofuels. 4: 12-.
Szijártó N, Horan E, Zhang J, Puranen T, Siika-aho M, Viikari L, (2011) Thermostable endoglucanases in the liquefaction of hydrothermally pretreated wheat straw. Biotechnology for Biofuels. 4: 2-.
Vepsäläinen L, Palmunen K, Uotila S, Visuri K, Rouvinen J, Kallio JM. (2011) Polysaccharides as precipitants in protein crystallization for X-ray diffraction studies. Crystal Growth & Design. 11, 4, 1152-1158. doi:10.1021/cg101359m.
Voutilainen SP, Boer H, Alapuranen M, Jänis J, Vehmaanperä J, Koivula A. (2009) Improving the thermostability and activity of Melanocarpus albomyces cellobiohydrolase Cel7B. Appl Microbiol Biotechnol. May;83(2):261-72. doi: 10.1007/s00253-008-1848-9. Epub 2009 Jan 16.
Voutilainen SP, Puranen T, Siika-Aho M, Lappalainen A, Alapuranen M, Kallio J, Hooman S, Viikari L, Vehmaanperä J, Koivula A. (2008) Cloning, expression, and characterization of novel thermostable family 7 cellobiohydrolases. Biotechnol Bioeng. Oct 15;101(3):515-28. doi: 10.1002/bit.21940.
Palmunen K, Visuri K. (2008) Protein crystallization. EBR – European Biopharmaceutical Review. Sep: 104-110.
Szijártó N, Siika-Aho M, Tenkanen M, Alapuranen M, Vehmaanperä J, Réczey K, Viikari L. (2008) Hydrolysis of amorphous and crystalline cellulose by heterologously produced cellulases of Melanocarpus albomyces. J Biotechnol. Sep 10;136(3-4):140-7. doi: 10.1016/j.jbiotec.2008.05.010. Epub 2008 May 28.
Voutilainen S, Puranen T, Siika-aho M, Kallio J, Hooman S, Viikari L, Vehmaanperä J, Koivula A. (2007) Cloning, expression and characterization of novel thermostable family 7 cellobiohydrolases. Biotechnol. Bioeng. 101: 515-528.
Benko Z, Drahos E, Szengyel Z, Puranen T, Vehmaanperä J, Réczey K. (2007) Thermoascus aurantiacus CBHI/Cel7A production in Trichoderma reesei on alternative carbon sources. Appl. Biochem. Biotechnol. 137-140(1-12): 195-204.
Voutilainen S, Boer H, Linder M, Puranen T, Rouvinen J, Vehmaanperä J, Koivula A. (2007) Heterologous expression of Melanocarpus albomyces cellobiohydrolase Cel7B, and random mutagenesis to improve its thermostability. Enz. Microb. Technol. 41: 234-243.
Viikari L, Alapuranen M, Puranen T, Vehmaanperä J, Siika-Aho M. (2007) Thermostable enzymes in lignocellulose hydrolysis. Adv Biochem Eng Biotechnol. 108:121-45. doi: 10.1007/10_2007_065.
Paloheimo M, Mäntylä A, Kallio J, Puranen T, Suominen P. (2007) Increased production of xylanase by expression of a truncated version of the xyn11A gene from Nonomuraea flexuosa in Trichoderma reesei. Appl. Environ. Microbiol. 73(10): 3215-3224.
Gamauf C, Marchetti M, Kallio J, Puranen T, Vehmaanperä J, Allmaier G, Kubicek CP, Seiboth B. (2007) Characterization of the bga1-encoded glycoside hydrolase family 35 beta-galactosidase of Hypocrea jecorina with galacto-beta-D-galactanase activity. FEBS J. 274: 1691-1700.
Paloheimo M, Mäntylä A, Kallio J, Puranen T, Suominen P. (2003) High-yield production of a bacterial xylanase in the filamentous fungus Trichoderma reesei requires a carrier polypeptide with an intact domain structure. Applied and Environmental Microbiology. 69: 7073-7082.
Chief Scientist, Founder
I have built my scientific and technological expertise in the development of microbial strains, platforms, and industrial enzymes. My focus has been and continues to be on enabling the efficient, industrially feasible cell-based production of high-quality biological products.
Working as an expert in a highly competent R&D team is rewarding. I believe that success lies in a consistent and structured way of working that supports innovation and growth.
“I’m a team player and enjoy brainstorming together with colleagues. I value trust, authenticity, and openness – supporting individual and organisational growth.“
CEO, Founder
I have had the privilege of leading highly successful teams in the industrial enzyme business that have developed competitive products for the mass market for 15 years. I have ensured business competitiveness by building an IP portfolio, and most importantly, by providing day-to-day stakeholder/customer support.
It should come as no surprise that technological development also inspires me. I have designed and led strategies, roadmaps and projects in terms of cell-based production platforms, Bio-IT and R&D digitalization.
“I want to be a value-adding leader that foster a culture of trust, collaboration and learning. I believe it enables our customers to reach their full potential and succeed beyond expectations.“
CTO, Founder
My technological foundation is built on the design of industrial crystallization processes for concentrated and purified products ranging from therapeutic proteins to enzymes. I am delighted to have been able to provide R&D services that are trusted by many, including some of the world’s largest pharmaceutical companies.
That decade was just an appetizer. It was followed by a deeper dive into industrial upscaling, particularly enzyme processing and formulation technologies, leading up to hundreds of commercial bulk enzyme products.
”Set a goal. My passion is to design the most high-quality, streamlined and cost-effective process to achieve the given goal.”